The invention provides a preparation method of a medicine for preventing and treating acute upper respiratory tract infection. The medicine is prepared from the following raw materials in percentage by mass: 5 to 25 percent of fructus forsythiae, 8 to 25 percent of honeysuckle flower, 3 to 12 percent of field mint, 8 to 25 percent of great burdock fruit, 10 to 20 percent of balloon flower root, 6to 15 percent of belamcandae root, 8 to 20 percent of isatis root, 8 to 20 percent of ledebouriella root, 8 to 20 percent of dryopteris root and 3 to 12 percent of licorice root. The raw medicines are extracted, concentrated, flocculated, clarified and then concentrated, and a preparation for preventing and treating acute upper respiratory tract infection is obtained under the condition of preserving the effect of raw medicines to a full extent. Proved by results of bacteriostatic tests on main pathogenic bacteria and pharmacology and toxicology tests on pain alleviation, inflammation resistance, and the like, the technology has the advantages of effectively enriching main active ingredients in raw medicines, reducing the losses of main active ingredients and improving antibacterial spectrum and bacteriostatic strength. The medicine effect, the safety and the reliability are better than those of a preparation made from the same raw materials with a traditional method. Meanwhile, preparation clarity can be improved, medicine occupation volume can be reduced, and the medicine is convenient to carry and convenient for children to take.