The invention discloses a double-layer moxifloxacin suppository and a preparation method thereof, which aim to provide a locally-applied suppository with quick response, good curative effect, nonirritant and minimal side effect. The double-layer suppository is divided into an inner layer and an outer layer, wherein the outer layer is a quick-release part which is quickly released to quickly reachoptimal blood concentration; and the inner layer is a sustained-release part which is actually an ordinary-release part not subjected to sustained-release treatment. The double-layer moxifloxacin suppository is applied from the vagina so as to avoid the damages of first pass effect of the liver to oral medication and the toxic or side effect of systemic administration, and make the effective medicament concentration of a local lesion greatly exceed levels obtained by oral medication and injection, has long effective duration and the curative effect remarkably better than that of the systemic administration, and is applied to the treatment of vaginitis caused by the infections of staphylococcus aureus, Chlamydia and the like.