The invention relates to a cyclone separator comprising a main body of the cyclone separator, a liquid discharging pipe, a solid discharging pipe and at least one sucking pipe, wherein one end of the liquid discharging pipe is positioned into the main body of the cyclone separator, the other end of the liquid discharging pipe passes through the top of the main body of the cyclone separator and ispositioned outside the main body of the cyclone separator; the solid discharging pipe is connected to the bottom of the main body of the cyclone separator and is communicated with the internal space of the main body of the cyclone separator; one end of the sucking pipe is communicated with a cyclone feeding hole in the main body of the cyclone separator, and the other end of the sucking pipe faces to the bottom direction of the main body of the cyclone separator; and the bottom of the solid discharging pipe is higher than the bottom of the sucking pipe. The invention also relates to a slurry-bed reaction and separation system, a material separating method for slurry-bed reaction and the application of the slurry-bed reaction and separation system in the Fischer-Tropsch synthesis technology. The technical scheme utilizing the cyclone separator, the separation system and the separation method in the invention can be used for realizing the solid-liquid separation of slurry materials inside a slurry-bed reactor.