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发明授权
CN102010880B 重型肝炎相关基因hFasmicroRNA腺病毒表达质粒的构建方法及其药学用途 失效 - 权利终止

重型肝炎相关基因hFasmicroRNA腺病毒表达质粒的构建方法及其药学用途
摘要：
重型肝炎相关基因hFas microRNA腺病毒表达质粒的构建方法及其药学用途，本发明涉及构建了一组基因生物制剂，即构建了重型肝炎相关的hFas基因的microRNA腺病毒表达质粒，其产生hFasmiRNA的单链寡核苷酸序列为：5′-TGCTGTTAAGTTGGAGATTCATGAGAGTTTTGGCCACTGACTGACTCTCATGACTCCAACCTTA-3′。在细胞水平检测microRNA干扰质粒的有效性和特异性，建立hFasmicroRNA腺病毒表达质粒应用于治疗重型肝炎。
摘要（英）：

The invention discloses a construction method and pharmaceutical application of hFas microRNA adenovirus expression plasmid relevant with severe hepatitis and relates to a method for constructing a group of gene biological preparations, i.e. the microRNA adenovirus expression plasmid of hFas gene relevant with severe hepatitis can be constructed. The single-stranded oligonucleotide sequence of the generated hFas microRNA is 5'-TGCTGTTAAGTTGGAGATTCATGAGTTTTGGCCACTGACTGACTCTCATGACTCCAACCTTA-3'. By using the invention, the effectiveness and the specificity of the microRNA interfering plasmid canbe detected in the cell level and the hFas microRNA adenovirus expression plasmid can be established and applied to treating the severe hepatitis.

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