The invention provides a method for measuring the contents of indole alkaloids in cinobufagin alcohol precipitation liquid. The alcohol precipitation method is a key unit operation in the production process of cinobufagin injection and a quick and effective content analysis method aiming at the alcohol precipitation process is especially important. The method comprises the following steps of: collecting a representative alcohol precipitation liquid sample as a correction set; measuring the contents of the indole alkaloids by adopting ultraviolet visible spectrophotometry and scanning a near-infrared original spectrum chart of the bisindole alkaloids at the same time; selecting a proper spectrum preprocessing method, a modeling spectrum section and a main factor number; and constructing a near-infrared quantitative correction model of the indole alkaloid of the alcohol precipitation liquid sample and quickly measuring the content of the cinobufagin alcohol precipitation liquid sample with the unknown content by using a chemometric method. The invention has the advantages of quickness, no damage and accuracy, can effectively solve the problems of lacking of quality monitoring and batch difference in the alcohol precipitation process and provides a new idea and means for quality control of the traditional Chinese medicine process.