The invention relates to a method for separating iron and zinc from high-iron sphalerit concentrate, and belongs to the technical fields of high-iron sphalerit concentrate metallurgy and beneficiation. The technical scheme comprises the following steps of: baking the high-iron sphalerit concentrate containing 35 to 48 weight percent of zinc, 7.5 to 25 weight percent of iron and 0.05 to 0.20 weight percent of indium to obtain hot baked sand; slowly cooling at a cooling rate of 1-15DEG C/min, and grinding and pulping the baked sand subjected to slow cooling treatment; and performing wet magnetic separation treatment to separate the zinc and iron ore from the baked sand so as to obtain low iron-zinc concentrate and indium-rich high iron-zinc concentrate. The method is short in process flow, easy to operate, easy to control, high in adaptability to raw materials, high in production efficiency, low in production cost, low in equipment investment, low in energy consumption, high in metal recovery and environment-friendly; and the iron and zinc can be efficiently separated from the baked sand prepared by baking the high-iron sphalerit concentrate.