The invention provides a breather mechanism of an air vibratory sputum excretion machine, belonging to the technical field of medical apparatus and instruments. The air vibratory sputum excretion machine comprises a shell with a rack, an air chamber in the shell, a power machine, an air induction mechanism, a tympanic membrane actuating mechanism and a breather pipe, wherein power machine is fixed on the bottom of the air chamber or the rack; the air induction mechanism is communicated with the air chamber and used for introducing air into the air chamber and fixed on the rack; the tympanic membrane actuating mechanism is arranged in the air chamber and connected with the power machine; and the breather pipe is connected with the air chamber and used for introducing air in the air chamberto a patient. The breather mechanism is characterized by comprising a first tympanic membrane tray and a second tympanic membrane tray, wherein the first and second tympanic membrane trays are oppositely arranged on the air chamber and connected with the tympanic membrane actuating mechanism. According to the technical scheme, as the first and second tympanic membrane trays are oppositely arranged on the air chamber and connected with the tympanic membrane actuating mechanism, the air in the air chamber shows a breathing-like effect under the working of the tympanic membrane actuating mechanism, and an ideal air vibration effect is obtained.