The invention discloses chryseobacterium sp. JT03 capable of using methane. The preservation number of a strain is CCTCC No.M2010100. The chryseobacterium sp. can be used for preparing a methane biological absorbent, a garbage treating agent and a biological catalyst. The chryseobacterium sp. overcomes the application defects of low bacteria density of methane-oxidizing bacteria, difficulty in amplification culture of bacteria and the like, and has high affinity to methane. The chryseobacterium sp. is particularly suitable for methane artificial sources such as household garbage landfill sites, paddy fields and the like, and can be widely popularized.