The invention discloses a secretory type alkaline phosphatase traced TA cloning eukaryon expression vector, wherein alkaline phosphatase traced TA cloning sites are inserted a downstream CMV and an upstream bovine growth hormone (BGH) poly-adenine transcription terminator. By adopting the vector of the invention, the supernatant of culture medium can be utilized for detection and the chemiluminescence instrument can be utilized for detection, thus the sensitivity is high and quantitative measurement can be realized by comparing with the standard alkaline phosphatase; the inventor changes the target gene insertion site to the TA cloning vector site, thus the polymerase chain reaction (PCR) product can be directly inserted in the reporter gene vector and the time and research cost can be reduced.