The invention discloses a method for transforming in-vitro soybean growing points with a gene gun, belonging to the technical field of bioengineering. The method is characterized by making bullets with carriers containing target genes, loading the bullets into the gene gun, then bombarding aseptic seedlings and performing inoculation induction to obtain T0-generation plants; performing rooting induction and seedling hardening by opening a vessel lid on the T0-generation plants, and then inducing the plants to bloom and fruit to obtain T1-generation seeds; and finally transplanting the T1-generation seeds to a phytotron or large fields to obtain T2-generation plants and homozygous positive transformant thereof. The method has the following advantage: by utilizing the characteristic that the plants prepared from the in-vitro soybean growing points are relatively easy to regenerate, the transformation efficiency can be obviously improved.