The invention relates to an alignment film printing method and device of a substrate of a liquid crystal display. The method comprises the following steps: acquiring the positional information of a substrate unit of one or more defective products of a substrate; sending the positional information to a process management system; transmitting the positional information to a first alignment film printing device by the process management system; and carrying out alignment film printing on the substrate units except the substrate unit of the defective product by virtue of the first alignment film printing device. The invention has the advantage that the positional information of the substrate unit of the defective product in the substrate is obtained to control the follow-up process to implement the steps of alignment film printing and inspection and the like on the substrate units expect the substrate unit of the defective product, thus the alignment film printing material cost is saved.