The invention discloses a dynamic parameter configuration-based general framing and de-framing method for serial port data frames, which comprises the following steps of: 1. performing conversion on data frames to be transmitted in a serial port unit according to frame format configuration parameters; 2. transmitting the converted data frames through the serial port unit; 3. receiving the converted data frames through the other serial port unit; and 4. performing de-framing on the data frames received by the other serial port unit according to the frame format configuration parameters. The data frames to be transmitted in the serial port units are matched in advance according to the preset frame format configuration parameters, the matched data frames to be transmitted are transmitted, and the received data frames are de-framed by the receiving serial port unit according to the preset frame format configuration parameters, therefore, the method can be applied to communication of the serial port units of different types; the invention provides the general method; and the method can also realize quick de-framing.