The invention discloses a hybrid seed production method for tobacco, which comprises the following steps of: planting a male parent and a female parent of the tobacco separately; adjusting a blooming stage by using a seedling stage to make the male parent blossoms 10-20 days earlier than the female parent; after the initial blooming stage of the male parent, picking flowers with a non-dehiscent anther everyday and collecting and storing pollen for later use; when a female parent plant is in a full-bloom stage, cutting fruits on the selected tobacco plant with only the flowers and buds left; tearing off blossomed corollas to expose stigmas; coating the pollen of the male parent on the stigmas of the female parent uniformly; and remaining 80-100 pollinated remaining fruits per plant. In themethod, links such as a planting manner, a planting ratio, the blooming stage, male parent pollen extraction time, a male parent extraction method, pollination time, a pollination method, a female parent fruit remaining number per plant and the like are improved, so that the efficiency and the quality of tobacco hybrid seed production are increased, the planting area of the male parents of the tobacco is reduced, the amount of labor used in artificial supplementary pollination of hybrid seed production is reduced, the pollination efficiency is increased, the seed production yield is increased, and the production cost of the hybrid seed production is reduced.