The invention relates to a bird nest fern spore germination breeding method which comprises the following steps of: wrapping a collected bird nest fern spore by using gauze, carrying out asepsis treatment and adding sterile water for blending into sterile spore turbid liquid containing 1,500-2,000 sterile spores per milliliter; mixing the sterile spore turbid liquid with an initial culture mediumaccording to the proportion of 1:4, wherein the initial culture medium is an MS culture medium in which 0.2-1.0mg of GA3, 20-60g of cane sugar and 6g of agar are added in each liter; after uniformly mixing, reserving a flat plate, wherein the thickness of the flat plate is 3-5mm; after solidifying, cutting into a sheet containing 600-800 spores in each part; charging the sheet into a culture dishin which an MS liquid culture medium is added for culturing, wherein the culture condition comprises the temperature of 23-25 DEG C and the illumination intensity of 1,500-2,000Lx; and illuminating for 16 hours each day till a spore seedling is formed.