The invention discloses a method for culturing a homozygous diploid carrot, comprising the following steps: (1) carrying out heat shock treatment on a carrot microspore; (2) culturing the carrot microspore subjected to the heat shock treatment to obtain an embryoid; and (3) carrying out tissue culture on the embryoid to obtain a regeneration plant of the carrot. Experiments prove that the method for culturing the homozygous diploid carrot, which is provided by the invention, has the advantages of higher embryoid forming rate and higher survival rate. The method is suitable for culturing carrots of various gene types; and by utilizing method, the stable homozygous diploid of the carrot can be quickly obtained, and the carrot breeding age limit is greatly shortened to improve the carrot breeding efficiency and facilitate prompting the carrot breeding process. The method disclosed by the invention has a wide application prospect in the field of carrot breeding.