The invention provides application of glucose to preparation of a magnesium diboride-doped superconducting material, and relates to the technical field of preparation of high-temperature superconducting materials. The preparation method comprises the following steps of: weighing magnesium powder and boron powder in a molar ratio of 1:(2-2.1) respectively; weighing glucose based on the ratio of the total mass of the magnesium powder and the boron powder to mass of the glucose of 1:(0.03-0.1); uniformly mixing the magnesium powder, the boron powder and the glucose powder to form mixed powder; sintering the mixed powder under the protection of argon atmosphere at the temperature of between 800 and 900 DEG C; and preserving heat for 0.5 to 2 hours to obtain the magnesium diboride-doped superconducting material in which the glucose is used as a carbon source. In the magnesium diboride superconducting material prepared by the method, critical current density is obviously improved, and particularly, the critical current density in a high magnetic field is extremely high; and the application of the material in the high magnetic field is facilitated, and the material has high practicability.