The invention discloses a Chinese medicine composition, in particular a Chinese medicine composition for treating cold of livestock and poultry and a preparation method thereof. The Chinese medicine composition for treating cold of livestock and poultry is characterized by being prepared from the following components: jasmine, pearl shell, buffalo horn, isatis root, dry rehmannia, radix scrophulariae, ophiopogonis radix, baicalin and liquorice. The Chinese medicine composition for treating cold of livestock and poultry is specially used for treating the cold of livestock and poultry and can fundamentally prevent and treat virus infection and comprehensively improve the virus resistance capacity of a body by adjusting the functions of humoral immunity, cellular immunity, nonspecifc immunity, and the like of the body. Compared with western medicines, the invention also has the advantages of low toxicity, no residue, low price, and the like.