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发明公开
CN102128929A 水稻矮缩病毒三抗夹心酶联免疫吸附检测试剂盒 无效 - 驳回

水稻矮缩病毒三抗夹心酶联免疫吸附检测试剂盒
摘要：
本发明公开了一种水稻矮缩病毒三抗夹心酶联检测试剂盒，试剂盒包含阳性对照、阴性对照、水稻矮缩病毒多克隆抗体、水稻矮缩病毒单克隆抗体、酶标抗体及其它材料和药品，所说的其它材料和药品包括被缓冲液、PBST、病毒抽提缓冲液、封闭缓冲液、底物缓冲液、底物、5块酶标板及20个一次性滴管，水稻矮缩病毒多克隆抗体在分离提纯水稻矮缩病毒后，通过家兔免疫分离血清得到；所述的水稻矮缩病毒单克隆抗体在分离提纯水稻矮缩病毒后，通过小鼠免疫、细胞融合、克隆纯化得到；本发明制备的试剂盒检测灵敏度高、特异性强，可规模化生产用于水稻矮缩病的田间监测。
摘要（英）：

The invention discloses a rice dwarf virus tri-anti sandwich enzyme-linked immuno sorbent assay kit which comprises positive control, negative control, a rice dwarf virus polyclonal antibody, a rice dwarf virus monoclonal antibody, an enzyme labeled antibody and other materials and drugs, wherein other materials and drugs comprise buffered solution, PBST, virus extraction buffer solution, sealing buffer solution, substrate buffer solution, a substrate, 5 enzyme label plates, and 20 disposable droppers; the rice dwarf virus polyclonal antibody is obtained through rabbit immune separation serum after separating and purifying a rice dwarf virus sugarcane separator; and the rice dwarf virus monoclonal antibody is obtained by mouse immune, cell fusion and clone purification. The kit prepared by the invention is high in detection sensitivity, strong in specificity, and suitable for large-scale production for field monitoring to the rice dwarf virus.

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