The invention provides a seed culturing method for improving colored leaf characteristics of acer rubrum varieties, belonging to the field of garden greening and biological propagation and cultivation. The method is characterized by comprising the following steps: screening varieties adapting to soil and climate conditions of southern areas from acer plants; performing a rootstock-scion combination test to select combination with higher survival rate and growing potential; measuring and sequencing the content of anthocyanin of the scion combination in a color changing phase; evaluating and sequencing the appreciation characteristics of the scion varieties; and screening the best rootstock acer buergerianum of the acer rubrum varieties based on comparison of the two sequences. The method has the advantages that the defects of unstable color changing effect and short colored appreciation period of the acer rubrum varieties in hot areas in autumn can be overcome; and inoculation is a common method for maintaining excellent characteristics of varieties in garden cultivation, but two plants are completely different in heredity are synthesized into a plant by utilizing the method provided by the invention, and the defect of undeveloped root of scion varieties is overcome by utilizing the advantage of strong adaptability of rootstock, so that the excellent characteristics of the gardening varieties are improved and the appreciation period is prolonged.