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发明公开
CN102140535A 预测重型肝炎病情基因甲基化状态检测方法及试剂盒 有权

预测重型肝炎病情基因甲基化状态检测方法及试剂盒
摘要：
本发明涉及预测重型肝炎病情基因甲基化状态检测方法及试剂盒，它设有对基因组DNA进行亚硫酸氢盐修饰的试剂，针对人谷胱甘肽S-转移酶M3基因启动子的甲基化特异性和非甲基化特异性的引物对，以及2×Taq PCRMasterMix，提取病人外周血单个核细胞DNA进行亚硫酸氢盐修饰后，分别用谷胱甘肽S-转移酶M3甲基化(M)特异性和非甲基化(U)特异性引物对进行甲基化特异性聚合酶链式反应，根据扩增产物判断其甲基化状态，有助于临床医生判定重型肝炎患者的病情和指导治疗。
摘要（英）：

The invention relates to a gene methylation state detection kit used for predicting the course of severe hepatitis. The kit contains a reagent used for performing hydrosulfite modification to the genomic DNA, a primer pair aiming at the methylation specificity and non-methylation specificity of the human glutathione S-transferase M3 promoter and 2*Taq PCRMasterMix. The detection method comprises the following steps: after hydrosulfite modification is performed to the extracted peripheral blood mono-nuclear cell DNAs of a patient, the primers of the glutathione S-transferase M3 methylation (M) specificity and non-methylation (U) specificity are used to perform methylation-specific polymerase chain reaction (PCR). According to the amplification product, the methylation state can be judged and the clinician can judge the course of severe hepatitis and guide the treatment.

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