The invention discloses a vibrio parahemolyticus dual-real-time fluorescence PCR (Polymerase Chain Reaction) detecting primer, a probe, a detecting kit and a detecting method. The primer, the probe and the reaction condition are optimized and designed by using a dual-real-time fluorescence PCR method and adopting a toxR gene and a tdh gene of vibrio parahemolyticus as target genes, so that the toxic gene is detected when specific detection is carried out on the vibrio parahemolyticus in an amplification reaction, the toxR gene is used for specifically detecting the vibrio parahemolyticus, andthe tdh gene is used for detecting whether a toxic gene is carried or not. The invention has the advantages of rapid detection, reliable result, high sensitivity and high specificity, can finish the process from sample preparation to detecting result issuing in 8 to 10 hours, is free from the inference of false positive gender, cross contamination, and the like, provides a favorable tool for carrying out epidemiological survey of vibrio parahemolyticus, is suitable for inspection and quarantine of foods and marine products and can enable an inspection and quarantine bureau, a disease prevention and control center and a quality supervision department to carry out simple, rapid and accurate detection on the sample.