The invention relates to a preparation method of fluorescent colorful offset paper, which comprises the steps of: completely changing the color of slurry into the required fluorescent yellow, fluorescent green, fluorescent red, fluorescent pink or fluorescent orange by utilizing several pigments, and then preparing the slurry into the fluorescent colorful offset paper according to the production procedures of finished papers of the conventional process. The preparation method of the fluorescent colorful offset paper is flexible, simple and convenient to operate and has a stable and reliable production process. The fluorescent colorful offset paper obtained through the preparation method disclosed by the invention has strong three-dimensional sense, a vivid color and an extremely good visual effect.