The invention relates to a portable liquid sampling box which has strong isolation and seal properties, is durable in use and is convenient to transport. The portable liquid sampling box comprises a box body on which a box cover is arranged; the box body is internally provided with circular groove barrels; the inner bottom wall of each circular groove barrel is connected with a spring; the upper end of each spring is connected with a supporting plate; the box body is divided into a plurality of layers, and every two adjacent layers are fastened together in the vertical direction; the box body is internally divided into a plurality of utensil tanks in which the circular groove barrels are arranged; the box body comprises a large bottle box, a small bottle box and a tool box, wherein the tool box is internally divided into a funnel area, a large tool area and a small tool area; the funnel area is internally provided with an iron case; the top wall of the box cover is internally connected with a buffer sponge; the inner side walls and the bottom wall of the box body are connected with buffer sponges; the inner side walls of the circular groove barrels are also connected with buffer sponges; a universal wheel and a directional wheel are fixed below the box body; an expansion handle is hinged on the box body through a hinge; elastic clamping slots are fixedly arranged on the side walls of the box body; and the expansion handle is clamped in the elastic clamping slots.