This invention discloses a coating conductor multilayered superconducting film and a preparation method thereof. The coating conductor multilayered superconducting film is YBa2Cu3Oy/Y0.9RE0.1Ba2Cu3Oy/Y0.7RE0.3Ba2Cu3Oy, wherein RE is Sm or Ho. The preparation method comprises the following steps of: preparing precursor liquid, coating the precursor liquid on each NiW/La2Zr207/CeO2 buffer layer; performing one-time pyrolysis for coating of each NiW/La2Zr207/CeO2 buffer layer; and crystallizing at high temperature to obtain multilayered superconducting film with thickness of 0.9 to 1.8 microns. By the invention, the directional controllable distribution of the defect structure in superconducting layer on the sample cross section can be realized, the pinning capacity of the interface and the thickness of the superconducting layer can be effectively increased and the performance of the superconducting layer is improved.