The invention discloses a solid substrate priming method of seeds of a seedless watermelon, relating to a seed treatment technology and comprising the following six steps of: firstly, selecting and pretreating a solid substrate, secondly, selecting and treating seeds, thirdly, priming the seeds, fourthly, separating the seeds from sawdust, fifthly, drying, and sixthly, storing the seeds at a low temperature. The method for conductivity measurement or activity measurement of dehydrogenase is adopted when the seeds are selected according to needs, the seeds with poor innate vigor are removed toavoid unnecessary priming waste. In the method, sawdust is used as the solid substrate, therefore, the material is easily available, the cost is low, the specific gravity and the friction coefficientare low, the operation is relatively convenient, the surface of a screen is less worn when in screening or separating, the seeds are cultured in darkness in an incubator so that electric power is saved, the priming effect of seeds is extremely ideal, and the positive effect on seed germination is superior to the priming effect of other solid substrates.