The invention discloses a method for inhibiting the trailing of a point of a solid-phase protein chip by changing a chemiluminescence mode, comprising the following steps of: adding a chemiluminescence substrate to carry out enzymatic reaction on the basis of adding a layer of buffer solution containing surface active agents by taking a point generated in the enzyme-immune chemiluminescence of the solid-phase protein chip as a research object; and then capturing an image. The protein chip prepared by adopting the method disclosed by the invention has strong uniformity and high detection accuracy; and the method has the advantages of low cost and easiness and convenience for operation.