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发明公开
CN102183644A 羊痘抗体间接ELISA诊断试剂盒及制备方法 无效 - 撤回

羊痘抗体间接ELISA诊断试剂盒及制备方法
摘要：
本发明公开一种能检测羊痘病毒的特异性抗体，可用于羊痘野毒感染的诊断和羊痘弱毒疫苗免疫后的抗体监测的ELISA诊断试剂盒。本发明的羊痘抗体间接ELISA诊断试剂盒，其中有包被抗原的ELISA抗体检测板和酶标二抗，抗体检测板上包被的抗原为羊痘病毒ORF122S重组蛋白，所用的二抗为兔抗山羊酶标二抗。本发明还公开了制备ORF122S重组蛋白的方法，即利用山羊痘病毒弱毒疫苗株基因组DNA为模板，用特异性引物进行PCR扩增，扩增产物经酶切后构建原核表达载体pET122S，再进行诱导表达。
摘要（英）：

The invention discloses an enzyme-linked immuno sorbent assay (ELISA) diagnostic kit capable of detecting specific antibody of capripox viruses and being used for diagnosis of capripox virus infection and antibody monitoring after immunization of weak capripox virus vaccines. The indirect capripox antibody ELISA diagnostic kit contains an antigen-coated ELISA antibody detection board and enzyme-labeled secondary antibody, wherein the antigen coated on the antibody detection board is capripox virus ORF122S recombinant protein, and the secondary antibody is rabbit anti-goat enzyme-labeled secondary antibody. The invention also discloses a method for preparing the ORF122S recombinant protein, namely genome DNA of weak capripox virus vaccine strains is used as a template, polymerase chain reaction (PCR) amplification is performed by using specific primers, the amplified product is digested by enzyme to construct a prokaryotic expression vector pET122S, and inducible expression is performed.

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