The invention discloses a strain of endometrial adenocarcinoma cell lines, wherein a name of the endometrial adenocarcinoma cell lines is LHY-821, and a preservation register number of the endometrial adenocarcinoma cell lines is CGMCC No.4066. The LHY-821 cell lines has advantages of uniform cell morphology, good cell growth, strong adhesive ability, doubling time of 44-48 hours and stable biological characteristics and genetic property. The LHY-821 cell lines can be applicable for research on biology of tumor cells, molecular biology, gene and epigenetics in vitro and in vivo, and on therapeutic effects of new antitumor drugs in a preclinical phase, and has a wide application prospect.