The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.