The invention relates to the field of food processing, in particular to a barley beta-glucan preparation method for extracting beta-glucan products from barley kernels endemic to Qinghai-Tibet Plateau. The preparation method comprises the following steps of: (1) riddling and crushing; (2) extracting; (3) centrifugally extracting; (4) acidifying; and (5) drying to obtain the beta-glucan products. The preparation method provided by the invention has the advantages that the adopted extracting technique is simple and is easy to operate, the required equipment is single, the used reagents are nontoxic, the waste materials generated in an extracting process can be used as stock food, the used ethanol can be recycled, water obtained after the ethanol is recovered can be reused, and no pollution is discharged in the whole extracting process, which is favorable for protecting the environment. The whole extracting process is free from danger, damage and pollution.