The invention provides a method and application of a photosynthetic bacteria culture solution in medicinal material planting. The method comprises the following steps: diluting the photosynthetic bacteria culture solution; and evenly irrigating soil, soaking seeds, irrigating roots and spraying. By utilizing the method, the medicinal material growth can be promoted; the occurrence of various disasters can be prevented and treated; the problem of manual culture quality reduction caused by utilizing chemical fertilizers and pesticides can be solved; the traditional Chinese medicine extract content can be increased; the content of the effective components in the traditional Chinese medicinal material is increased; the heavy metal content is reduced; the utilization of the Chinese medicinal material is improved; the sustainable utilization of the Chinese medicinal material is realized; the resources are protected; the implementation of GAP (good agriculture practice) in China can be improved; the problem that crop rotation can not be performed in Chinese medicinal material planting can be solved; and the fertilizer amount is saved by one third.