The invention discloses a bacterial strain for preventing mildew of stored flue-cured tobacco strips, and belongs to the field of biological control of plant diseases. The strain has been collected in China General Microbiological Culture Collection Center on August 23, 2010 with the collection number of CGMCC No.4116, and is classified and named Bacillus pumilus. The strain can inhibit the growth of mould of the flue-cured tobacco strips, has good control effect on the mildew caused by the mould, and comprises an active ingredient of a whole culture solution culture or spores of the Bacilluspumilus LZ-7. The bacterial strain has the advantages of low production and use cost, convenience in use, good anti-mildew effect, promotion of alcoholization of the flue-cured tobacco strips and thelike, and overcomes the defects that a chemical mildewproof agent easily influences the quality of the flue-cured tobacco strips, easily causes residue and the like.