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发明公开
CN102251015A 环脂酸芽孢杆菌显色培养基及其使用方法和用途 失效 - 权利终止 许可

环脂酸芽孢杆菌显色培养基及其使用方法和用途
摘要：
本发明涉及用于检测环脂酸芽孢杆菌的显色培养基及其使用方法和用途，该培养基包含A组分、B组分以及C组分，其中，所述A组分包含以重量份计的下述物质：酵母提取物2至4份，葡萄糖5至10份，CaCl2.2H2O0.25至0.4份，MgSO4.7H2O 0.3至0.5份，(NH4)2SO4 0.1至0.3份，KH2PO4 2.5至3.5份，所述B组分包含以重量份计的下述物质：琼脂15至20份，β-半乳糖苷酶底物0.08至0.12份，所述C组分为1个重量份，该C组分包含以重量份计的下述物质：ZnSO4.7H2O 0.18×10-3至0.2×10-3份，CuSO4.5H2O0.16×10-3至0.18×10-3份，MnSO4.H2O 0.15×10-3至0.17×10-3份，COCl2.6H2O0.18×10-3至0.2×10-3份，H3BO3 0.1×10-3至0.12×10-3份，NaMoO4.2H2O0.3×10-3至0.5×10-3份。本发明的显色培养基对环脂酸芽孢杆菌具有良好的生长支持能力，并且能够以高特异性检测出目标菌。
摘要（英）：

The invention relates to a chromogenic culture medium for detecting alicyclobacillus as well as application method and use thereof. The chromogenic culture medium comprises a component A, a component B and a component C, wherein the component A comprises the following substances in parts by weight: 2-4 parts of a yeast extract, 5-10 parts of glucose, 0.25-0.4 part of CaCl2.2H2O, 0.3-0.5 part of MgSO4.7H2O, 0.1-0.3 part of (NH4)2SO4 and 2.5-3.5 parts of KH2PO4; the component B comprises the following substances in parts by weight: 15-20 parts of agar and 0.08-0.12 part of beta-galactosidase substrate; and 1 part by weight of the component C comprises the following substances in parts by weight: 0.18*10<-3>-0.2*10<-3> part of ZnSO4.7H2O, 0.16*10<-3>-0.18*10<-3> part of CuSO4.5H2O, 0.15*10<-3>-0.17*10<-3> part of MnSO4.H2O, 0.18*10<-3>-0.2*10<-3> part of COCl2.6H2O, 0.1*10<-3>-0.12*10<-3> part of H3BO3 and 0.3*10<-3>-0.5*10<-3> part of NaMoO4.2H2O. The chromogenic culture medium providedby the invention has strong capability of supporting growth of the alicyclobacillus and can be used for detecting the target alicyclobacillus with high specificity.

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