The invention relates to a quality detection method for tobacco leaves and application thereof, and belongs to the field of the analytical detection of tobacco leaf raw materials. In a sampling and sample preparation method for detecting the inherent quality of the tobacco leaf raw materials by a half leaf process, half of laminae of the tobacco leaves are used as detection samples according to the natural characteristic that the laminae of the tobacco leaves are in bilateral symmetry about an axis of chief veins or tobacco stems, so the source quantity of the samples is improved by one time correspondingly under the condition of the same detection sample number, the sampling quantity is smaller, labor and materials are saved during sampling, and the accuracy of detection results can be ensured. Simultaneously, when the method is applied to a contrast experiment of the physical quality evaluation of the tobacco leaf raw materials, the fine change of the tobacco leaf samples processed under a certain experimental condition is found by comparing two sides of the laminae of the same tobacco leaves, so that the change law of the tobacco leaf samples can be mastered accurately.