The invention discloses a glutelin GluB-4 gene terminator of a rice seed and application thereof. The terminator is NDA molecules being the following molecules (1) or (2) or (3); the molecules (1) are DNA molecules consisting of nucleotide sequences shown in the sequence 1 of a sequence table; the sequence of DNA molecules restricted by the molecules (2) and the molecules 1) has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% of homology molecules; the molecules (3) are hybrid molecules of the DNA sequence restricted by the molecules (1) or the molecules (2) in strict conditions. With the use of matching the terminator provided by the invention with different promoters, the expression and accumulation level of exogenous genes in the objective plants can be improved, a foundation is laid for the upgrading seed quality with biotechnology and researching molecular medicine farms and the like, andthe application prospect is extremely large.