The invention relates to the field of fermentation, in particular to a strain of high temperature resistant Issatchenkia orientalis, application thereof and a method for producing ethanol through fermentation. The strain of high temperature resistant Issatchenkia orientalis IPE100 is separated and screened from farmland crop waste, has a number of CGMCC (China General Microbiological Culture Collection Center) No.4927, has the capacity of fermenting ethanol at high temperature, and can be applied to high temperature fermentation of ethanol by using lignocelluloses hydrolysis products and hightemperature solid fermentation of ethanol by using sweet sorghum.