The invention provides a preparation method of poly2,5-furandimethyl glycol ester. The method comprises steps that: 2,5-furandimethyl and aliphatic dibasic alcohol are subject to an esterification reaction under the effect of a catalyst quadrivalent titanium compound, such that an esterified product is obtained; the esterified product is subject to a condensation polymerization reaction, such that poly2,5-furandimethyl glycol ester is obtained. According to the invention, the quadrivalent titanium compound catalyst with a relatively high activity is used as the catalyst for the esterificationreaction and the condensation polymerization reaction, no additional catalyst is required in the condensation polymerization reaction, such that the operation is simplified. Also, the preparation method provided by the invention is characterized by short production period and high production efficiency. Therefore, the method is suitable for industrialized productions. As results of experiments, poly2,5-furandimethyl glycol ester provided by the invention has a reduced viscosity equaling to or greater than 0.2dL/g, a vitrification temperature of 28 DEG C to 60 DEG C, and a melting point of 145DEG C to 214 DEG C.