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CN102277453A 罗氏沼虾双顺反子病毒的LAMP检测试剂盒及检测方法 有权

罗氏沼虾双顺反子病毒的LAMP检测试剂盒及检测方法
摘要：
罗氏沼虾双顺反子病毒的LAMP检测试剂盒及检测方法，属于靶DNA片断的检测技术领域。该检测试剂盒包括RNA提取试剂和RT-LAMP反应试剂，该检测方法包括1）罗氏沼虾RNA抽提；2）罗氏沼虾双顺反子病毒的RT-LAMP扩增：3）显色检测。该检测试剂盒特异性强，敏感性高，该检测方法方便、灵敏、准确、快速，为罗氏沼虾幼体综合症的预防奠定基础。
摘要（英）：

The invention discloses an LAMP detection kit and an LAMP detection method for macrobrachium rosenbergii bicistronic virus, which belongs to the technical field of target DNA segments. The detection kit comprises an RNA extraction reagent and a reverse transcription (RT)-LAMP reaction reagent. The detection method comprises: 1) extracting RNA of macrobrachium rosenbergii; 2) performing the RT-LAMP amplification of the macrobrachium rosenbergii bicistronic virus; and 3) performing color developing detection. The detection kit has high specificity and sensitivity, the detection method is convenient, sensitive, accurate and quick, and thus, a foundation is laid for the prevention of syndrome in larvae of macrobrachium rosenbergii.

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