The invention relates to an anti-biological clogging device for a drip-irrigation system. The drip-irrigation system is started, an air intake brake valve is opened, an ozone generator is started, and compressed water which comes from the head part of the drip-irrigation system is sprayed at high speed from a jet orifice of a jet nozzle so as to form the negative pressure in a gas injection cavity; and under the action of the negative pressure, the ozone generator ensures that ozone generated through air ionization enters the gas injection cavity through the air intake brake valve, a check valve and an air inlet pipe along with the air filtered by an air filter, and enters an oscillating cavity for vortex oscillation, stirring and aerification along with the high-speed water flow sprayed by the jet nozzle so as to form 'ozone water' of which gas and liquid are distributed uniformly, and the 'ozone water' enters the drip-irrigation system to kill microorganisms in flow channels and cansynchronously inhibit crop root systems from growing toward the direction of a water outlet of a drip irrigation emitter in an underground drip-irrigation system, so that the aim of biological clogging resistance of the drip-irrigation system is fulfilled. Compared with a method of injecting a chemical agent into irrigation water, the invention has the advantages that: the residue of harmful substances which can pollute the environment is not produced, and the risk of using dangerous chemical agents is avoided.