The invention discloses a method for measuring the content of benzene and benzene series in hot melt glue. The method comprises the following steps of: selecting benzene, methylbenzene, ethylbenzene, o-xylene, p-xylene or m-xylene as standard substances of the benzene and the benzene series, and gradually diluting to form a standard working solution by taking glyceryl triacetate as a matrix correction fluid; taking a hot melt glue sample and adding the hot melt glue sample into the matrix correction fluid, and detecting and analyzing the standard working solution and the sample by using a headspace gas chromatography mass spectrometry; and finally, drawing a standard curve and calculating a result. In the invention, a complex pretreatment process of the sample, and influences and interference caused by volatile components in the sample are eliminated. According to the method, the recovery rate ranges from 95% to 101%, the average relative standard deviation ranges from 3.04% to 4.14%, and the repeatability is good.