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CN102329896A 一种水生双顺反子病毒的RT-PCR检测方法 无效 - 驳回

一种水生双顺反子病毒的RT-PCR检测方法
摘要：
一种水生双顺反子病毒的RT-PCR检测方法，属于病毒检测技术领域。其包括以下步骤：1）用水生双顺反子病毒特异性引物P0对待测RNA样品进行逆转录得到cDNA第一链，cDNA用水生双顺反子病毒通用引物组P1/P0进行PCR扩增；2）上述得到的扩增产物用电泳鉴定；并且本发明设计了特异性引物P0、通用引物组P1/P0和通用引物组P2/P3。本发明能同时检测桃拉综合症病毒、锯缘青蟹双顺反子病毒和罗氏沼虾双顺反子病毒基因组。本发明直观优越、敏感性好、特异性高，对检测微量、保存时间久的标本中水生双顺反子病毒基因组的效果良好，可用于水生双顺反子病毒感染的实验室检测和水生双顺反子病毒的分子流行病学调查。
摘要（英）：

The invention provides a RT-PCR (reverse transcription-polymerase chain reaction) detection method of aquatic bicistronic virus, belonging to the technical field of virus detection. The method comprises the following steps: 1) carrying out reverse transcription on a RNA sample to be detected with a specific primer P0 of aquatic bicistronic virus to obtain a first chain of cDNA, and carrying out PCR amplification on cDNA with a universal primer group P1/P0 of aquatic bicistronic virus; and 2) identifying the obtained amplified products with electrophoresis. In the invention, the specific primer P0, the universal primer group P1/P0 and a universal primer group P2/P3 are designed. By using the method in the invention, taura syndrome virus, scylla serrata bicistronic virus, M. rosenbergii bicistronic virus genomes can be simultaneously detected. The method is intuitionistic and superior, has good sensitivity and high specificity as well as good detection effect on the aquatic bicistronic virus genome in a trace long-storage sample, and can be used for lab detection of aquatic bicistronic virus infection and molecular epidemiological survey of aquatic bicistronic virus.

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