The invention discloses a stem cell bank with a liquid nitrogen gas-liquid separation device on a liquid nitrogen delivery pipeline. The stem cell bank comprises a plurality of independent stem cell storage tanks and a pipe which is used for providing liquid nitrogen to the stem cell storage tanks. The pipe is connected with an external liquid nitrogen tank. An electromagnetic valve is arranged at the tail end of the pipe. A temperature sensor is arranged on the internal side of the pipe adjacent to the electromagnetic valve. The temperature sensor and the electromagnetic valve are respectively connected with an intelligent controller. The intelligent controller controls the opening and the closing of the electromagnetic valve according to data transmitted by the temperature sensor. By using an automatic control system to control the liquid nitrogen gas-liquid separation on the nitrogen delivery pipeline, the pressure in the pipe is kept normal, the gas is exhausted out of the stem cell bank in time, the outflow of the liquid nitrogen caused by human factors is reduced, and the situations that a safety valve jumps up, the liquid nitrogen is spilt outwards and personnel injuries are caused can be avoided. Since the device is not operated manually, the safety of liquid nitrogen filling personnel of the stem cell bank is protected.