The invention discloses a method for separating and purifying Centella asiatica triterpene acid monoglucoside by using crude Centella asiatica triterpene acid monoglucoside as the raw material. The method comprises the following steps: carrying out column chromatography on crude Centella asiatica triterpene acid monoglucoside, eluting using a mobile phase containing beta-cyclodextrin, collecting fractions respectively, and concentrating and drying to obtain asiatic acid monoglucoside, chebuloside II and madecassic acid monoglucoside. By adopting the technical scheme of the invention, pure Centella asiatica triterpene acid monoglucoside can be separated from crude Centella asiatica triterpene acid monoglucoside. In addition, the method is simple to operate, has lower cost and is suitable for large-scale production and applications.