The invention provides a preparation method for a rhesus ovary and ovarian follicle generation model. According to the method, female rhesus monkeys are used as test objects, and each rhesus monkey is injected with 60 IU of luteinizing hormone LH each time and is injected twice everyday for seven days successively to prepare the rhesus ovary and ovarian follicle generation model. The invention also provides application of luteinizing hormone LH in preparing medicines for activating ovary and stimulating ovarian follicle thereof to grow. According to the invention, monkeys are used in the method for preparing the model, background data in physiology, hematology, blood biochemistry and the like of monkeys are abundant and easily available, monkeys have a biological background resembling to human beings, and therefore, test results of monkeys can well indicate clinical reactions of medicines; multi-aspect and multi-angle assessment are carried out, and obtained results can well and objectively indicate drug action on a reproductive system; animals (monkeys) and instruments (a B-mode ultrasonoscope, a microscope, a cell culture device, etc.) used in the model are simple and easily available, and the model has versatility.