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发明授权
CN102399903B 一种基孔肯雅病毒等温扩增检测试剂盒及其引物 失效 - 权利终止

一种基孔肯雅病毒等温扩增检测试剂盒及其引物
摘要：
本发明提供了一种基孔肯雅病毒等温扩增检测试剂盒及其引物，所述试剂盒包括引物、Bst DNA聚合酶、反应液和SYBR Green I荧光染料；所述反应液为10mM脱氧核苷三磷酸、10×反应缓冲液和150mM MgSO4；所述引物序列是如SEQ ID NO：1-4所示的序列，或如SEQID NO：5-8所示的序列，或如SEQ ID NO：9-12所示的序列，或如SEQ ID NO：13-16所示的序列。本发明的试剂盒检测时间短、特异性强、灵敏度高、检准率高、反应程序化、鉴定简便，广泛应用于临床、口岸的常规检测及流行病学调查。
摘要（英）：

The invention provides a chikungunya virus isothermal amplification detection kit and a primer thereof. The kit comprises the primer, Bst DNA polymerase, a reaction liquid and SYBR Green I fluorescent dye, wherein the reaction liquid is 10mM deoxyribonucleoside triphosphate, 10*reaction buffer liquid and 150mM MaSO4; the sequences of the primer are sequences represented by SEQ ID NO:1-4 or sequences represented by SEQ ID NO:5-8 or sequences represented by SEQ ID NO:9-12 or sequences represented by SEQ ID NO:13-16. The kit provided by the invention has the advantages of short detection time, strong specificity, high sensitivity, high detection accuracy and programmed reaction, is easy and convenient to qualify and is widely applied to conventional detection and epidemiological survey in clinic and ports.

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