The invention provides a method for separating tin, antimony, bismuth, arsenic and copper from tin electrolytic anode mud. The method comprises the following steps of: grinding the tin electrolytic anode mud finely, mixing the tin electrolytic anode mud with an oxidizing agent, adding a NaOH solution, stirring the mixture at a certain temperature under certain pressure, and thus obtaining dissolved serous fluid; and filtering to obtain a sodium stannate solution, cooling naturally to room temperature, filtering, decomposing the obtained filtrate by introducing carbon dioxide or adding NaHCO3, filtering, and thus obtaining an alkali solution and stannic oxide. By the method, the problem of difficulty in separation of the tin and the antimony in the tin anode mud is solved, and other valuable metals in the tin anode mud can be subsequently recovered smoothly; compared with a method for processing the anode mud by an acid method, the method has the obvious advantage that: the discharging of waste liquid and waste gas is avoided in the process of utilizing the anode mud comprehensively; and the method has a short process, makes simple equipment used, is energy-saving and environment-friendly and is worthy of being applied and popularized.