The present invention provides reference protein rice heat shock 90 protein suitable for rice western blotting research. The rice heat shock 90 protein is stably expressed in the materials of the rice with different development periods and different tissues, in the materials of xanthomonas oryzae pv. oryzae at different time, and in the materials of the rice at different times of day and night, such that the rice heat shock 90 protein can be adopted as the reference protein for the rice western blotting research. The present invention further provides monoclonal antibody corresponding to the rice heat shock 90 protein. With adopting the protein fragment expressed in vitro as the immunogen to immunize Balb/c mice, the resulting hybridoma XXX secrets to obtain the Rice90-31 monoclonal antibody, wherein the antibody can specifically identify the recombinant protein and the rice natural protein, and the use titer in the experiment is 1:10000-1:20000. The linear detection range of the prepared Rice90-31monoclonal antibody of the present invention is 0.41-8.2 ng, the lowest detection limit of the prepared Rice90-31monoclonal antibody in the rice protein is about 0.24 ng, and the HSP protein content in the total rice protein is about 0.12% by using the standard curve.