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CN102465160A 一种诱变赤灵芝原生质体选育高产胞外多糖菌株的制备方法 无效 - 撤回

  • 专利标题： 一种诱变赤灵芝原生质体选育高产胞外多糖菌株的制备方法
  • 专利标题（英）： Method for breeding high-yield extracellular polysaccharide bacterial strain by ganoderma lucidum protoplast mutagenesis
  • 申请号： CN201010535869.8
    申请日： 2010-11-04
  • 公开(公告)号： CN102465160A
    公开(公告)日： 2012-05-23
  • 发明人: 王刚强
  • 申请人： 王刚强
  • 申请人地址： 江苏省徐州市云龙区民祥园14号楼5-301
  • 专利权人： 王刚强
  • 当前专利权人： 王刚强
  • 当前专利权人地址： 江苏省徐州市云龙区民祥园14号楼5-301
  • 主分类号： C12P19/04
  • IPC分类号： C12P19/04
一种诱变赤灵芝原生质体选育高产胞外多糖菌株的制备方法
摘要：
本发明涉及一种灵芝多糖的制备方法，具体是涉及一种诱变赤灵芝原生质体选育高产胞外多糖菌株的制备方法，属遗传技术领域。它以赤灵芝为菌种，经马铃薯葡萄糖琼脂培养基活化、淀粉液体摇瓶培养后，利用溶壁酶、蜗牛酶和纤维素酶水解赤灵芝菌丝体，在恒温下振荡，制备赤灵芝原生质体。诱变菌株经过传代后，胞外多糖含量比原发菌株提高了6～8倍，且遗传性状较稳定，该工程菌株可用于工业化生产灵芝多糖。
摘要（英）：

The invention relates to a preparation method of a mutagenic ganoderma polysaccharide, specifically, relates to a method for breeding a high-yield extracellular polysaccharide bacterial strain by ganoderma lucidum protoplast mutagenesis, and belongs to the technical field of the hereditism. The method utilizes ganoderma lucidum as a strain and comprises the following steps of carrying out activation by a potato dextrose agar medium, carrying out shake-flask culture in starch liquid, carrying out ganoderma lucidum mycelium hydrolysis by lywallzyme, helicase and cellulose, and carrying out shaking at a constant temperature to obtain a ganoderma lucidum protoplast. Through passage, the mutagenic high-yield extracellular polysaccharide bacterial strain has extracellular polysaccharide content which is 6 to 8 times higher than extracellular polysaccharide content of the original bacterial strain. The high-yield extracellular polysaccharide bacterial strain has stable hereditary, is an engineering strain and can be utilized for ganoderma lucidum polysaccharide industrial production.

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