The invention discloses a user authentication method based on keystroke mode identification, which comprises the steps that: firstly, the normal user keystroke training is carried out, the keystroke characteristic feature of users is extracted, and a single classifier is used for training the keystroke mode of the users; the users log in and input passwords, a system obtains the keystroke information of the users, and the information comprises user names, the passwords and the keystroke characteristic feature of the users; the current input passwords and passwords in a user mode base are subjected to password mode matching by an authentication module of an operation system; and finally, the user keystroke information verification is carried out, an interface enters an application system or the operation system if the verification is passed, and the interface returns for logging again if the verification is not passed. The user authentication method has the advantages that the safety performance of the system can be effectively improved, the keystroke behavior of the users is adopted for user authentication, and a certain protection effect can still be ensured after the passwords of the users are stolen.