The invention discloses an insecticidal composition containing cycloxaprid and biological insecticide. The first active ingredient is the cycloxaprid; the second active ingredient is the biological insecticide, namely abamectin, emamectin benzoate, spinosad or spinetoram; and the weight ratio of the first active component to the second active component is 1:5-30:1. The composition can be preparedinto agriculturally allowable water dispersible granules, wettable powder, suspension, missible oil or micro emulsion. The composition has reasonable ingredients, a good insecticidal effect and low administration cost, and the activity and the insecticidal effect of the composition are not simply superposed activity and insecticidal effect of each component; compared with a single preparation, the composition has a remarkable synergistic effect and good safety besides the remarkable insecticidal effect and meets the safety requirement of pesticide preparations; and the insecticidal composition has a good preventing and controlling effect on pests such as wheat aphids, citrus aphids, cotton aphids, blind stinkbugs, rice plant hoppers, vegetable thrips and the like.